RHCE Membrane Protein Introduction

Introduction of RHCE

The blood group Rh(CE) polypeptide is a membrane protein encoded by the RHCE gene in humans. It is also known as Rh polypeptide 1 (RhPI), Rh30A, RhIXB, Rhesus C/E antigens. RHCE has also recently been designated as CD240CE (cluster of differential 240 CE). RHCE has four major alleles encoding a combination of Ce, Ce, Ce, and Ce antigens, 4. The RHD and RHCE genes, each consisting of 10 exons, represent a group of genes. The Rh blood group system is the second most important in the blood group, second only to the ABO blood group. It is also one of the most polymorphic types of blood, with mutations due to deletions, gene conversions, and erroneous mutations. The gene encoding the RhC and RhE antigens on a single polypeptide is a member of the Rh blood group. The group also includes the gene which encodes the RhD protein. According to the presence or absence of highly immunogenic RhD proteins on the erythrocyte surface, the classification of Rh-positive and Rh-negative individuals is determined. Selective splicing of this gene results in four different forms of transcript variants.

Basic Information of RHCE
Protein Name	Blood group Rh(CE) polypeptide
Gene Name	RHCE
Aliases	Rh polypeptide 1 (RhPI), Rh30A, RhIXB, Rhesus C/E antigens, CD_antigen: CD240CE
Organism	Homo sapiens (Human)
UniProt ID	P18577
Transmembrane Times	11
Length (aa)	417
Sequence	MSSKYPRSVRRCLPLWALTLEAALILLFYFFTHYDASLEDQKGLVASYQVGQDLTVMAALGLGFLTSNFRRHSWSSVAFNLFMLALGVQWAILLDGFLSQFPPGKVVITLFSIRLATMSAMSVLISAGAVLGKVNLAQLVVMVLVEVTALGTLRMVISNIFNTDYHMNLRHFYVFAAYFGLTVAWCLPKPLPKGTEDNDQRATIPSLSAMLGALFLWMFWPSVNSPLLRSPIQRKNAMFNTYYALAVSVVTAISGSSLAHPQRKISMTYVHSAVLAGGVAVGTSCHLIPSPWLAMVLGLVAGLISIGGAKCLPVCCNRVLGIHHISVMHSIFSLLGLLGEITYIVLLVLHTVWNGNGMIGFQVLLSIGELSLAIVIALTSGLLTGLLLNLKIWKAPHVAKYFDDQVFWKFPHLAVGF

Function of RHCE Membrane Protein

RHCE may be part of an oligomeric complex that is likely to have a transport or channel function on the erythrocyte membrane. A recent study of the population of Sardinia showed that in the RHCE gene (rs630337), a non-coding variant was associated with erythrocyte sedimentation rate (ESR). This suggests a possible causal effect of this polymorphism on this inflammatory marker, although not found in the coding region of the gene.

RHCE Membrane Protein Introduction Fig.1 The structure of Blood group Rh(CE) polypeptide.

Application of RHCE Membrane Protein in Literature

Kappler-Gratias S., et al. Systematic RH Genotyping and Variant Identification in French Donors of African Origin. Blood Transfusion. 2014, 12 (Suppl 1): s264-s272. PubMed ID: 23867180

In 1 (0.3%) and 17 (5%) cases, a rare Rh phenotype was predicted from RhCE molecular analysis, associated with a high prevalence antigen or a portion of RhCE antigen.
Fichou Y., et al. Insights into RHCE Molecular Analysis in Samples with Partial D Variants: The Experience of Western France. Transfusion Medicine and Hemotherapy. 2015, 42 (6): 372-377. PubMed ID: 26733768

According to the results of this study, the authors suggest that all individuals with weak genotype D 4.2.2 should be screened for RHCE variants by the conventional molecular analysis system.
Chou S.T., et al. Whole-Exome Sequencing for RH Genotyping and Alloimmunization Risk in Children with Sickle Cell Anemia. Blood Advances. 2017, 1 (18): 1414-1422. PubMed ID: 29296782

These results suggest that RH allelic variation in SCA patients is clinically relevant, and NGS technology can provide a comprehensive alternative to SNP-based testing.
Gaspardi A.C., et al. Clinically Relevant RHD-CE Genotypes in Patients with Sickle Cell Disease and in African Brazilian Donors. Blood Transfusion. 2016, 14 (5): 449-454. PubMed ID: 27177398

These results suggest that knowledge of the distribution and prevalence of RH alleles in SCD patients and African-American donors is important for projects that implement RH genotype matching in SCD patients who have RH alleles and Clinically significant RH antibodies.
Westhoff C., et al. RHCE*ceMO Is Frequently in Cis to RHD*DAU0 and Encodes a hr^S-, hr^B-, RH:-61 Phenotype in Blacks; Clinical Significance. Transfusion. 2013, 53 (1102): 10.1111/trf.12271. PubMed ID: 23772606

The authors conclude that antibodies to transformed patients depend on the trans RHCE allele

RHCE Preparation Options

To obtain the soluble and functional target protein, the versatile Magic™ membrane protein production platform in Creative Biolabs enables many flexible options, from which you can always find a better match for your particular project. Besides, aided by our versatile Magic™ anti-membrane protein antibody discovery platform, we also provide customized anti-RHCE antibody development services.

Creative Biolabs' skillful scientists are glad to leverage our expertise and advanced technologies to help you with the member protein research. If you are interested, please feel free to contact us for more details.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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